Journal: Oncogene

Article Title: Tyrosine phosphorylation of C-Cbl facilitates adhesion and spreading while suppressing anchorage-independent growth of V-Abl-transformed NIH3T3 fibroblasts.

doi: 10.1038/sj.onc.1202672

Figure Lengend Snippet: Figure 3 The eect of c-Cbl overexpression on formation of focal adhesion complexes and actin ®bers in v-Abl-transformed NIH3T3 cells. Cells overexpressing Tyr5-4Phe (a and e) or wild-type c-Cbl (b, c, d, f, g) were ®xed and stained with anti-Cbl (a ± g), phalloidin-Texas Red (a ± d) and anti-paxillin (e ± g) as described in Materials and methods. An LSM410 confocal microscope (Zeiss) and the manufacturer's software were used to analyse cell images. Anti-Cbl rabbit polyclonal IgG and anti-paxillin mouse mAb were visualized using secondary antibodies labeled with FITC (green) and Texas Red, respectively. No staining was detected with irrelevant antibodies (not shown). The results of an independent representative experiment from a total of three experiments are shown

Article Snippet: Anti-Cbl and anti-paxillin were followed, respectively, by the a nitypuri®ed donkey anti-rabbit IgG conjugated to FITC and the a nity-puri®ed donkey anti-mouse IgG conjugated to Texas Red (Jackson ImmunoResearch Laboratories, West Grove, PA, USA).

Techniques: Over Expression, Transformation Assay, Staining, Microscopy, Software, Labeling